Resources
Stem cells, Protocols and Plasmids
Stem Cells:
Protocols:
NIH Center for Alzheimer's and Related Dementias (Link)
Lentiviral transduction of iPSCs with sgRNAs and sgRNA libraries (Link)
iNeuron pre-differentiation & differentiation protocol V.2 (Link)
WTC11 iPSC Culture and Maintenance (Link)
Differentiation of iPSCs with the NGN2 construct into cortical neurons protocol (Link)
Plasmids:
Online Resources & Public Datasets:
The Myeloid Landscape 2 (Link)
Brain RNA-Seq (Link)
Microglia Gene List Enrichment Calculator (Link)
ABC Atlas (Link)
Human Cell Atlas Explorer (Link)
The Brain Immune Atlas (Link)
CZ CELLxGENE (Link)
Single Cell Portal (Link)
Panglao DB (Link)
The Mouse Brain Atlas (Link)
NeuroLipid Atlas (Link)
Aging, Dementia and TBI study (Link)
Conferences & Webinars:
European Microglia Webinar Series (Link)
Online Seminars:
Marius Wernig: Next generation cell therapies for the brain @ NHGRI DIR Seminar Series (Link)
Beth Stevens #1: Microglia States in Health and Disease (Link)
Beth Stevens #2: How Microglia Sculpt Brain Circuitry in Health and Disease (Link)
Beth Stevens: Redefining Microglia States and Function in Alzheimer’s Disease (Link)
Bess Frost: Transposable Element Activation in Neurodegenerative Tauopathy: From Bench to Bedside (Link)
David Holtzman: Sleep & the Pathophysiology of Alzheimer's Disease (Link)
Ben Barres: What do reactive astrocytes do? (Link)
Bo Peng: Microglia replacement: from bench to clinical therapy (Link)
Marco Prinz: The myeloid side of brain (Link)
Martin Kampmann: Multiplexing with CRISPR Screens (Link)
Jennifer Doudna: CRISPR-Cas9: From Biology to Transformative Technology (Link)
Anna Molofsky: Regulation of microglial function by cytokines (Link)
Anne Schaefer: Negative feedback control of neuronal activity by microglia (Link)
Lindsay De Biase: Basal ganglia microglia shape circuit function across the lifespan (Link)